[1]冯毅,马静,黄贞.牙髓干细胞促进牙髓血管生成的相关分子机制研究[J].牙体牙髓牙周病学杂志,2013,23(12):768-772.
 FENG Yi*,MA Jing,HUANG Zhen.Underlying mechanism of human dental pulp stem cell- induced angiopoiesis[J].Chinese journal of conservative dentistry,2013,23(12):768-772.
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牙髓干细胞促进牙髓血管生成的相关分子机制研究
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《牙体牙髓牙周病学杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
23
期数:
2013年12期
页码:
768-772
栏目:
论著
出版日期:
2013-12-31

文章信息/Info

Title:
Underlying mechanism of human dental pulp stem cell- induced angiopoiesis
作者:
冯毅1马静1黄贞2
1. 榆林市星元医院口腔科,陕西 榆林 719000; 2. 第四军医大学口腔医学院, 陕西 西安 710032
Author(s):
FENG Yi* MA Jing HUANG Zhen
*Department of Stomatology, Xingyuan Hospital, Yulin 719000, China
关键词:
牙髓干细胞 血管再生 VEGF PI3K/AKT MAPK
Keywords:
human dental pulp stem cells(HDPSCs) angiopoiesis VEGF PI3K/AKT MAPK
分类号:
R780.2
文献标志码:
A
摘要:
目的: 探讨牙髓干细胞(HDPSCs)是否具有在体外促进血管再生的潜能及其分子机理。方法:体外分离培养HDPSCs,经表面标志物检测鉴定后,用RT-PCR、Western blot、ELISA分别检测细胞内及其培养基中血管生成相关因子(vascular endothelial growth factor, VEGF)、内皮抑素、尿激酶型纤溶酶原激活物(u-PA)、白介素8(IL- 8)、基质细胞衍生因子1α(SDF-1α)的转录和蛋白表达水平;同时利用MTT法和Transwell小室实验分别测定HDPSCs条件培养液培养12~72h后血管内皮细胞的增殖和迁移能力,Western blot检测信号通路在血管内皮细胞中的活化状态。 结果:HDPSCs中VEGF和u- PA表达水平较高,与之相比,IL-8和SDF-1α的表达水平较低,而内皮抑素的表达水平最低。HDPSCs条件培养液可促进血管内皮细胞的增殖,这种效应可被VEGF和SDF-1α抗体逆转。HDPSCs也可诱导血管内皮细胞发生迁移,同时阻断血管内皮细胞中PI3K/AKT和MAPK信号通路可显著抑制其迁移能力。结论:HDPSCs可通过分泌血管生成相关因子促进血管内皮细胞的增殖和迁移,血管内皮细胞中PI3K/AKT和MAPK信号通路可能参与了血管再生的过程。
Abstract:
AIM: To investigate whether human dental pulp stem cells (HDPSCs) play roles in promoting angiopoiesis in dental pulp and to elucidate the underlying mechanism.METHODS:Primary HDPSCs were cultured in vitro and characterized by their membrane molecular biomarkers. The expression of cellular and secreted angiopoiesis- related factors including VEGF, endostatin, u- PA, IL- 8, SDF- 1α and SDF- 1α in culture supernatant was detected using RT- PCR, Western blotting and ELISA respectively. MTT test and Transwell assay were applied to evaluate the effects of HDPSCs on the proliferation and migration of human microvascular endothelial cells (HMECs). Cellular signaling pathway activation was detected by Western blotting. [WT5”HZ]RESULTS: [WT5”BZ]In HDPSCs high levels of VEGF and u- PA expression were found. IL- 8 and SDF- 1α showed lower expression, whereas, anti- angiopoiesis factor endostatin showed the least level. The HDPSCs conditioned medium promoted the proliferation of HMECs, which was reversed by the antibodies to VEGF and SDF- 1α. In addition, HDPSCs induced the migration of HMECs. Furthermore, inhibition of PI3K/AKT and MAPK pathways in HMECs could significantly surpress the migration of HMECs. CONCLUSION: HDPSCs may promote HMEC proliferation and migration by secretion of angiopoiesis- related factors. PI3K/AKT and MAPK pathways in HMECs may be involved in angiopoiesis of HMECs.

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更新日期/Last Update: 2013-12-30