[1]张亚男,邹朝晖,靳晓兰,等.光动力疗法对牙骨质龋抑制作用的实验研究[J].牙体牙髓牙周病学杂志,2013,23(12):753-756.
　ZHANG Ya- nan*,ZOU Zhao- hui,JIN Xiao- lan,et al.Inhibiting effect of photodynamic therapy on cementum caries[J].Chinese journal of conservative dentistry,2013,23(12):753-756.
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光动力疗法对牙骨质龋抑制作用的实验研究
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《牙体牙髓牙周病学杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
23
期数:
2013年12期
页码:
753-756
栏目:
论著
出版日期:
2013-12-31

文章信息/Info

Title:
Inhibiting effect of photodynamic therapy on cementum caries
作者:
张亚男1; 邹朝晖1; 靳晓兰1; 阴慧娟2
天津 300070: 1. 天津医科大学口腔医学院; 2. 中国医学科学院生物医学工程研究所
Author(s):
ZHANG Ya- nan*;  ZOU Zhao- hui;  JIN Xiao- lan ;  YIN Hui-juan
*Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
关键词:
光动力疗法;  根面龋;  活性氧
Keywords:
photodynamic therapy;  root caries;  reactive oxygen species
分类号:
R781
文献标志码:
A
摘要:
目的:观察光动力疗法(photodynamic therapy，PDT)对牙骨质龋的抑制效果。方法：将50个牙骨质块与粘性放线菌、变异链球菌和乳酸杆菌的混合菌液共同培养24d，成功建立牙骨质早期龋模型后随机分为5组（单纯光敏剂组、单纯激光照射组、PDT组、洗必泰阳性对照组、生理盐水阴性对照组，n=10）。按分组进行相应处理后，采用平板菌落计数观察杀菌效果；然后再将各组样本置于新鲜培养液中进行培养，分别于培养8、16、24、32、40、48h各时间点取各组培养液，用原子吸收光谱法测量其中的钙离子含量。结果：与阴性对照组相比，PDT可使牙骨质龋样本中的致龋菌数量显著减少(P<0.05),其杀菌率为98.70％，明显高于其他各组（P<0.05）；各组钙离子溶出量均随培养时间延长而增加，其中以PDT组的增加幅度最小，各时间点的钙离子溶出量均显著低于其他各组(P<0.05)。结论：光动力疗法对牙骨质龋有一定的抑制作用。
Abstract:
AIM: To observe the inhibiting effect of photodynamic therapy (PDT) on cementum caries. METHODS: 50 cementum blocks were cultured with mixed suspension of A. Viscosus, S. mutans and L. acidophilus for 24 d to develop caries. The blocks with caries were then divided into 5 groups(n=10): photosensitizer group (L-P+), laser group(L+P-), PDT group(L+P+), 0.5g/L chlorhexidine group (positive control) and normal saline group (negative control ). After respective treatment, the cementum blocks were put into 1 mL saline and stirred for 3 min. the solutions were then serially diluted and cultured in plates for 48 h. Bacteriacidal effects of the treatment was measured by colony- forming unit (CFU) counting. The blocks were further put into 6 mL culture medium and cultured anaerobically. At 8 h, 16 h, 24 h,32 h,40 h and 48 h of culture, 1 mL suspension was withdrawn and subjected to atomic absorption spectrometry for the measurement of the calcium ion content. RESULTS: The bacterialcidal effect of PDT was 98.70％, which was significantly higher than that of photosensitizer(1.23%, P<0.05), laser(56.96%, P<0.05), positive control(60.62%, P<0.05) and negative control(0%, P<0.05). Calcium dissolution increased with time in all groups. However, PDT group showed the least calcium dissolution (P<0.05). CONCLUSION: PDT may inhibit the develpment of cementum caries.

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更新日期/Last Update: 2013-12-30