[1]晏燕,陈先卓,周云,等.白细胞介素-1β通过P38MAPK信号通路调节人牙周膜成纤维细胞MMP-1表达的研究[J].牙体牙髓牙周病学杂志,2013,23(12):748-752.
 YAN Yan*,CHEN Xian- zhuo,ZHOU Yun,et al.IL-1β modulates matrix metalloproteinase- 1 expression in humanperiodontal ligament fibroblasts via P38MAPK pathway[J].Chinese journal of conservative dentistry,2013,23(12):748-752.
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白细胞介素-1β通过P38MAPK信号通路调节人牙周膜成纤维细胞MMP-1表达的研究
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《牙体牙髓牙周病学杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
23
期数:
2013年12期
页码:
748-752
栏目:
论著
出版日期:
2013-12-31

文章信息/Info

Title:
IL-1β modulates matrix metalloproteinase- 1 expression in humanperiodontal ligament fibroblasts via P38MAPK pathway
作者:
晏燕12陈先卓2周云1壮荣1曹军1
1. 第四军大学口腔医学院, 陕西 西安 710032; 2. 川北医学院附属医院, 四川 南充 637000
Author(s):
YAN Yan* CHEN Xian- zhuo ZHOU Yun ZHUANG Rong CAO Jun
*School of stomatology, The Fourth Military Medical University, Xi'an 710032, China
关键词:
白细胞介素-1β 人牙周膜成纤维细胞 P38丝裂原活化蛋白激酶 基质金属蛋白酶1
Keywords:
IL- 1β human periodontal ligament fibroblasts P38MAPK MMP- 1
分类号:
R780.2
文献标志码:
A
摘要:
目的: 探讨P38丝裂原活化蛋白激酶(P38 mitogen- activated protein kinase,P38MAPK)信号通路在白细胞介素-1β(IL- 1β)调节人牙周膜成纤维细胞中基质金属蛋白酶-1(MMP- 1)表达中的作用。方法:体外分离培养的人牙周膜成纤维细胞(hPDLFs),随机将其分为:①对照组:加入等量无血清培养液;②实验组1:加入10ng/mL IL- 1β作用24h;③实验组2:加入10μmol /L SB203580预处理30min后,再加入10ng/mL IL- 1β作用24h,然后分别采用噻唑蓝比色测定法(MTT)检测各组hPDLFs的增殖情况;Real- time PCR、免疫荧光技术及Western blot观察MMP- 1各组mRNA和蛋白表达的变化。结果:各组MMP- 1mRNA和蛋白的表达均以IL- 1β作用组最高, IL- 1β联合SB203580复合作用组次之,对照组最低,3组间两两比较,差异均具有统计学意义(P<0.05)。结论:IL- 1β可通过激活p38MAPK途径促进人牙周膜成纤维细胞MMP-1的表达。
Abstract:
AIM: To investigate the effects of IL- 1β on the expression of matrix metalloproteinase-1(MMP-1)in human periodontal ligament fibroblasts(hPDLFs)via P38 mitogen- activated protein kinase(P38MAPK) pathway.METHODS:hPDLFs were isolated and cultuered in vitro, and then were randomly assigned to 3 groups.① In control group hPDLFs were cultured without any stimulus;② in IL- 1β group hPDLFs were incubated with 10ng/mL of IL- 1β for 24h; ③ in IL- 1β+SB203580 group hPDLFs were pretreated for 30 min with the P38MAPK specific inhibitor SB203580 at 10μmol/L,and then treated with 10ng/mL of IL- 1β for 24h. The cell proliferation was examined by MTT assay, and MMP- 1 expression was detected by RT- PCR, immuofluorenscence and Western blot. RESULTS:In the 3 groups cell proliferation activity was similar. The expression of MMP- 1 mRNA and protein was the highest in the IL- 1β group, moderate in IL- 1β+SB203580 group and the lowest in control group(berween each 2groups, P<0.05).CONCLUSION: IL- 1β can promote MMP- 1 expression in human periodontal ligament fibroblasts via P38MAPK pathway.

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更新日期/Last Update: 2013-12-30