[1]褚云娟,叶菁,李晶,等.SDF- 1α/CXCR4轴影响牙周膜干细胞血管内皮向分化的初步研究[J].牙体牙髓牙周病学杂志,2013,23(12):743-747.
 CHU Yun- juan,YE Jing,LI Jing,et al.Effects of SDF-1α/CXCR4 axis on the endothelial differentiation of periodontal stem cells[J].Chinese journal of conservative dentistry,2013,23(12):743-747.
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SDF- 1α/CXCR4轴影响牙周膜干细胞血管内皮向分化的初步研究
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《牙体牙髓牙周病学杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
23
期数:
2013年12期
页码:
743-747
栏目:
论著
出版日期:
2013-12-31

文章信息/Info

Title:
Effects of SDF-1α/CXCR4 axis on the endothelial differentiation of periodontal stem cells
作者:
褚云娟叶菁李晶王萌王新文陈芳王欣刘玲侠王勤涛
第四军医大学口腔医学院, 陕西 西安 710032
Author(s):
CHU Yun- juan YE Jing LI Jing WANG Meng WANG Xin Wen CHEN Fang WANG Xing LIU Ling-xia WANG Qing-tao
School of Stomatology , The Fourth Military Medical University , Xi'an 710032 , China
关键词:
牙周膜干细胞 炎症 基质细胞衍生因子-1α 趋化因子受体4 内皮向分化
Keywords:
periodontal ligament stem cells inflammation stromal cell- derived factor- 1α chemokine(c- x- c motif)receptor4(CXCR4) endothelial differentiation
分类号:
R780.2
文献标志码:
A
摘要:
目的:探讨牙周炎时SDF- 1α/CXCR4轴对PDLSCs血管内皮向分化的影响。方法:分别从正常和炎症牙周组织分离培养鉴定牙周膜干细胞(H- PDLSCs、P- PDLSCs);Real time PCR检测两种干细胞中基质细胞衍生因子-1α(stromal cell-derived factor- 1α,SDF- 1α)及趋化因子受体4[chemokine(c- x- c motif)receptor4,CXCR4]的表达;然后用SDF- 1α分别对两种干细胞进行诱导(14d),Real time PCR检测H- PDLSCs和P- PDLSCs中成血管相关基因CD31、VWF mRNA的表达; Matrigel基质胶管腔形成实验观察、比较H- PDLSCs和P- PDLSCs的管腔形成情况及差异。结果: P- PDLSCs较H- PDLSCs高表达CXCR4 mRNA (P<0.05),低表达SDF- 1αmRNA(P<0.05);经SDF- 1α诱导后,P- PDLSCs中成血管相关基因CD31、VWF mRNA的表达水平均明显高于H-PDLSCs(P<0.05);其管腔形成数量也明显高于H- PDLSCs (P<0.05)。结论:P- PDLSCs中CXCR4 mRNA高表达,对牙周炎症组织环境中的SDF- 1α更加敏感,其血管内皮向分化的趋势和能力均增强。
Abstract:
AIM: To investigate the effects of SDF- 1α/CXCR4 axis on the endothelial differentiation of pexiodontal ligament stem cells(PDLSCs). METHODS: The stem cells from the periodontal ligament tissues of healthy subjects (H- PDLSCs) and periodontitis patients(P- PDLSCs) were isolated and cultured. Real time PCR were used to examine the expression of stromal cell- derived factor- 1α(SDF- 1α) and chemokine(c- x- c motif)receptor4(CXCR4) in H- PDLSCs and P- PDLSCs. The expression of endothelial gene CD31 and VWF in H- PDLSCs and P- PDLSCs after inclubation with SDF- 1α was tested using real time PCR. H- PDLSCs and P- PDLSCs were induced by SDF- 1α and seeded on matrigel, the capillary network and the tube formation were observed under phase contrast microscope.RESULTS:The expression of CXCR4 mRNA in P- PDLSCs was higher than in H- PDLSCs(P<0.05), but the expression of SDF- 1α mRNA in P- PDLSCs was lower (P<0.05). After inclubation with SDF- 1α the expression of CD31 and VWF in P- PDLSCs was higher than in H- PDLSCs (P<0.05). Both H- PDLSCs and P- PDLSCs after inclubation with SDF- 1α were able to create the capillary network and there were more tube formation in P- PDLSCs than in H- PDLSCs(P<0.05). CONCLUSION: The expression of CXCR4 mRNA in P- PDLSCs is higher than in H- PDLSCs. P- PDLSCs are more sensitive to SDF- 1α and have stronger capability of endothelial differentiation.

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更新日期/Last Update: 2013-12-30