[1]丁芳,吴家媛,贾谦.TGF- β1 对人根尖牙乳头干细胞增殖和分化的影响[J].牙体牙髓牙周病学杂志,2011,21(07):375-379.
 DING Fang,WU Jia-yuan,JIA Qian,et al.The effect of TGF-β1 on the proliferation and differentiation of human stem cells from the apical papilla[J].Chinese journal of conservative dentistry,2011,21(07):375-379.
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TGF- β1 对人根尖牙乳头干细胞增殖和分化的影响
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《牙体牙髓牙周病学杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
21
期数:
2011年07期
页码:
375-379
栏目:
论著
出版日期:
2011-07-31

文章信息/Info

Title:
The effect of TGF-β1 on the proliferation and differentiation of human stem cells from the apical papilla
作者:
丁芳 吴家媛 贾谦
1:遵义医学院附属口腔医院, 贵州 遵义 563000; 2:第四军医大学口腔医院, 陕西 西安 710032
Author(s):
DING Fang WU Jia-yuan JIA Qian LIU LiYI Guo-mei
Hospital of Stomatology, Zunyi Medical College, Zunyi 563003, China
关键词:
[关键词] 转化生长因子TGF-β1 干细胞 根尖牙乳头 增殖 分化
Keywords:
[Key words] transforming growth factor- beta1 stem cells apical papilla proliferation differentiation.
分类号:
R780.2
文献标志码:
A
摘要:
[摘要]目的: 研究转化生长因子 (TGF- β1 ) 在体外对根尖牙乳头干细胞(SCAP ) 增殖和分化的影响。 方法: 采用酶消化法获得原代培养人SCAP,并对获得克隆化的SCAP进行免疫组化染色和多向分化能力的初步鉴定。用 MTT比色法观察不同浓度的TGF- β1 对细胞增殖情况,并通过检测ALP活性和矿化诱导后COL1、DSPP、OCN mRNA表达,分析TGF- β1 对SCAP分化能力的影响。结果:5、10、20、40 ng/ mL组的TGF- β1 在SCAP培养1周内可显著促进细胞增殖(P<0.05 );在TGF-β1 作用8d 后, 细胞ALP活性增强(P<0.05);SCAP矿化诱导2周后,TGF-β1 组中COL1、OCN、DSPP的mRNA表达上调(P<0.05 ) 。 结论: TGF-β1 促进SCAP细胞增殖,并通过提高 ALP活性和上调COL1,OCN、DSPP mRNA的表达促进SCAP分化。 
Abstract:
[Abstract] AIM: To investigate the effect of transforming growth factor- beta 1(TGF-β1) on the proliferation and differentiation of human stem cells from the apical papilla (SCAP) in vitro. METHODS: SCAPs were primarily cultured with enzyme-digestion method and identified with immunohistochemical staining and multipotent differentiation. MTT assay, alkaline phosphatase (ALP) activity assay and RT- PCR for COL1,OCN and DSPP were used to detect the effect of TGF- β1 on proliferation and differentiation of SCAP. RESULTS: TGF- β1 dose-dependently increased SCAP proliferation during the 1 w culture period(P<0.05). TGF-β1 enhanced ALP activity after 8 d culture (P<0.05). Two weeks after culture in osteo/dentinogenic medium, the expressions of COL1,OCN and DSPP in SCAP cells were significantly increased(P<0.05). CONCLUTION: TGF-β1 can increase SCAP proliferation and differentiation by up-regulating ALPase activity and the expression of COL1,OCN and DSPP.
更新日期/Last Update: 2011-07-30